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The cell lysates were solubilized in RIPA buffer containing 1 mM sodium orthovanadate, 1 mM sodium fluoride, 10 mM N-ethylmaleimide, 5 g/mL leupeptin, 5 g/mL aprotinin, and 2 mM phenylmethylsulphonyl fluoride, after which they were immunoprecipitated with FLAG beads

Insoluble debris was removed by a 1 h centrifugation at 200,000 x g

Smooth muscle cells (SMCs) and stem cells (SSCs) share similar characteristics to tumor cells: chromosomal abnormalities, excessive proliferation, lack of apoptosis, invasiveness, and rapid growth

Carotenoids inhibit proliferation and regulate expression of peroxisome proliferators-activated receptor gamma (PPAR) in K562 cancer cells

If vitamin C is already part of your routine, you may also want to explore our Liposomal Vitamin C formula for another bioavailable option